mini rotofor chamber  (Bio-Rad)

Journal: Applied microbiology and biotechnology

Article Title: Cloning and high-level production of a chitinase from Chromobacterium sp. and the role of conserved or nonconserved residues on its catalytic activity.

doi: 10.1007/s00253-006-0614-0

Figure Lengend Snippet: Fig. 9 One-step purification of Chi54 and its mutated chitin- ases. a Silver staining after SDS-PAGE with culture super- natant of recombinant E. coli (lane 0) and samples obtained in each fraction of the Rotofor cell (lanes 6–20). b Silver staining after SDS-PAGE with samples obtained in fractions 18–20, where lanes 1–7 indicate pro- teins of Chi54, T218S, I270V, I270L, N401V, V493M, and S497E, respectively, and M indicates the molecular weight marker

Article Snippet: The samples were loaded in a Mini Rotofor chamber (BioRad, Hercules, California) and run at 12 W constant power for 4 h with 0.1 M H3PO4 at the anode and 0.1 M NaOH at the cathode.

Techniques: Purification, Silver Staining, SDS Page, Recombinant, Molecular Weight, Marker

Journal:

Article Title: Two Nonoverlapping Domains on the Norwalk Virus Open Reading Frame 3 (ORF3) Protein Are Involved in the Formation of the Phosphorylated 35K Protein and in ORF3-Capsid Protein Interactions

doi: 10.1128/JVI.77.6.3569-3577.2003

Figure Lengend Snippet: Purified 23K and 35K forms of the ORF3 protein. Sf9 cells were infected with ORF3NHA baculovirus recombinant. The ORF3NHA protein was purified by extraction with 1.5% CHAPS and preparative IEF with a Bio-Rad Rotofor. The purified ORF3NHA protein was resolved by SDS-18% PAGE and Coomassie blue R-250 staining. 35K band A and 23K band B were sequenced by using MALDI-TOF mass spectrometry.

Article Snippet: The fractions containing proteins with pIs between 4.5 and 4.8 were pooled and refocused by using a Bio-Rad 18-ml mini Rotofor chamber at a constant power of 12 W with 0.5% ampholytes (pH range, 3 to 5) as described above.

Techniques: Purification, Infection, Recombinant, Staining, Mass Spectrometry